IL-15RA Polyklonaler Antikörper

IL-15RA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16744-1-AP

Synonyme

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

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  WB analysis using 16744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16744-1-AP (IL-15RA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

16744-1-AP bindet in WB, IHC, IF, ELISA IL-15RA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-15RA fusion protein Ag10151
• Vollständiger Name: interleukin 15 receptor, alpha
• Berechnetes Molekulargewicht: 231 aa, 24 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC107777
• Gene symbol: IL-15RA
• Gene ID (NCBI): 3601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights have been reported (PMID: 10480910; 15976182; 15671076; 17912462).

Publikationen

Species	Application	Title
human	IHC	Nat Commun; An Artificial Intelligence-guided signature reveals the shared host immune response in MIS-C and Kawasaki disease.; Authors - Pradipta Ghosh
human	WB	Clin Sci (Lond); HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.; Authors - Jing Li
human	IHC	Stem Cells Dev; Human gastric cancer mesenchymal stem cell-derived IL15 contributes to tumor cell EMT via up-regulation of Tregs ratio and PD-1 expression in CD4+T cell.; Authors - Li Sun
human	IHC	Immunology; Interleukin-15 receptor α expression in inflammatory bowel disease patients before and after normalization of inflammation with infliximab.; Authors - Perrier Clémentine C
human,hamster	IHC	bioRxiv; AI-guided discovery of the invariant host response to viral pandemics.; Authors - Debashis Sahoo
human	IHC	EBioMedicine; AI-guided discovery of the invariant host response to viral pandemics; Authors - Debashis Sahoo