IL-17A Polyklonaler Antikörper

IL-17A Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(62)

Kat-Nr. : 13082-1-AP

Synonyme

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, interleukin 17A

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  IHC staining of human colon cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13082-1-AP (IL-17A antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13082-1-AP (IL-17A antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13082-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13082-1-AP (IL-17A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Kolongewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 34 publications below
• IF: See 12 publications below
• FC: See 1 publications below

Produktinformation

13082-1-AP bindet in WB, IHC, IF, ELISA IL-17A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Kaninchen
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Ag3733
• Vollständiger Name: interleukin 17A
• Berechnetes Molekulargewicht: 155 aa, 18 kDa
• GenBank-Zugangsnummer: BC067505
• Gene symbol: IL-17A
• Gene ID (NCBI): 3605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. This antibody got 32-35 kDa band in western blot, maybe due to homodimer formation and differential glycosylations.

Publikationen

Species	Application	Title
	WB	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
	WB	Int J Biol Macromol; Chitosan-modified dihydromyricetin liposomes promote the repair of liver injury in mice suffering from diabetes mellitus; Authors - Qiteng Ding
human	IF	J Autoimmun; IL-17 is expressed on beta and alpha cells of donors with type 1 and type 2 diabetes.; Authors - Sakthi Rajendran
	WB	J Med Chem; Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease.; Authors - Ji-An Chen
	IHC	J Invest Dermatol; Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing.; Authors - Rodero Mathieu P MP
	IF	Arch Oral Biol; Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium.; Authors - Koichiro Irie