IL-17A Polyklonaler Antikörper
IL-17A Polyklonal Antikörper für IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF, FC, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13082-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Magenkrebsgewebe, humanes Zervixkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 11 publications below |
| IHC | See 22 publications below |
| IF | See 7 publications below |
| FC | See 1 publications below |
Produktinformation
13082-1-AP bindet in WB, IHC, IF, FC, Cell treatment, ELISA IL-17A und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | IL-17A fusion protein Ag3733 |
| Vollständiger Name | interleukin 17A |
| Berechnetes Molekulargewicht | 155 aa, 18 kDa |
| GenBank-Zugangsnummer | BC067505 |
| Gene symbol | IL17A |
| Gene ID (NCBI) | 3605 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. This antibody got 32-35 kDa band in western blot, maybe due to homodimer formation and differential glycosylations.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| IHC protocol for IL-17A antibody 13082-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Autoimmun IL-17 is expressed on beta and alpha cells of donors with type 1 and type 2 diabetes. | ||
J Med Chem Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease. | ||
J Invest Dermatol Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing. | ||
Arch Oral Biol Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium. | ||
Cell Physiol Biochem Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis. | ||
J Cell Mol Med Human breast milk-derived exosomes through inhibiting AT II cell apoptosis to prevent bronchopulmonary dysplasia in rat lung. |
