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  • KD/KO Validated

IL-17A Monoklonaler Antikörper

IL-17A Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(34)

CloneNo.

1B3D5

Kat-Nr. : 66148-1-Ig

Synonyme

IL 17, IL17, IL-17, IL17A, 1B3D5



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Raji-Zellen, Ramos-Zellen, RAW 264.7-Zellen, U-937-Zellen
Erfolgreiche Detektion in IF-Phumanes Tonsillitisgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66148-1-Ig bindet in WB, IHC, IF-P, ELISA IL-17A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-17A fusion protein Ag3733
Vollständiger Name interleukin 17A
Berechnetes Molekulargewicht 155 aa, 18 kDa
Beobachtetes Molekulargewicht 18 kDa
GenBank-ZugangsnummerBC067505
Gene symbol IL-17A
Gene ID (NCBI) 3605
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-17A antibody 66148-1-IgProtokoll herunterladen
IF protocol for IL-17A antibody 66148-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Clin Immunol

Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia

Authors - Mengting Liu
mouseWB,IHC

Int J Pharm

Microneedle system carrying Momordin Ic-loaded ROS-responsive hydrogel ameliorates psoriasis via targeted anti-inflammatory and reactive oxygen species (ROS)-scavenging mechanisms

Authors - Chang Wang
mouseWB

Mol Nutr Food Res

A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways

Authors - Qianye Zhang
mouseIHC

J Integr Med

Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9

Authors - Ya Zhong
mouseWB

Int Immunopharmacol

(RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis.

Authors - Liangjun Deng
humanWB

Int Immunopharmacol

IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.

Authors - Yachun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Rosa (Verified Customer) (07-08-2022)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: adipose tissue
IL-17A Antibody Immunofluorescence validation (1:150 dilution) in adipose tissue (Cat no:66148-1-Ig)