IL-17A Monoklonaler Antikörper

IL-17A Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

CloneNo.

1B3D5

Rezensionen (1)

Kat-Nr. : 66148-1-Ig

Synonyme

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, interleukin 17A

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Galerie der Validierungsdaten

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  WB analysis using 66148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66148-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66148-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66148-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66148-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66148-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66148-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 66148-1-Ig

  1X10^6 Jurkat cells were stained with 0.2ug IL-17A antibody (66148-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Raji-Zellen, Ramos-Zellen, RAW 264.7-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Produktinformation

66148-1-Ig bindet in WB, IHC, IF, FC, ELISA IL-17A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Ag3733
• Vollständiger Name: interleukin 17A
• Berechnetes Molekulargewicht: 155 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC067505
• Gene symbol: IL17A
• Gene ID (NCBI): 3605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

Publikationen

Species	Application	Title
mouse	WB	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
mouse	IHC	J Integr Med; Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9; Authors - Ya Zhong
mouse	WB	Int Immunopharmacol; (RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis.; Authors - Liangjun Deng
human	WB	Int Immunopharmacol; IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.; Authors - Yachun Li
human	IHC	Biomed Pharmacother; P-STAT3 and IL-17 in tumor tissues enhances the prognostic value of CEA and CA125 in patients with lung adenocarcinoma.; Authors - Xiao-Ke Chen
human,mouse	WB,IHC	J Ethnopharmacol; Khasianine ameliorates psoriasis-like skin inflammation and represses TNF-α/NF-κB axis mediated transactivation of IL-17A and IL-33 in keratinocytes.; Authors - Yixi Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rosa (Verified Customer) (07-08-2022)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: adipose tissue

IL-17A Antibody Immunofluorescence validation (1:150 dilution) in adipose tissue (Cat no:66148-1-Ig)