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IL-17A Monoklonaler Antikörper
IL-17A Monoklonal Antikörper für WB, IF-P, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF-P, ELISA
Konjugation
Unkonjugiert
CloneNo.
1B3D5
Kat-Nr. : 66148-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Raji-Zellen, Ramos-Zellen, RAW 264.7-Zellen, U-937-Zellen |
| Erfolgreiche Detektion in IF-P | humanes Tonsillitisgewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 25 publications below |
| IHC | See 5 publications below |
| IF | See 9 publications below |
Produktinformation
66148-1-Ig bindet in WB, IHC, IF-P, ELISA IL-17A und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | IL-17A fusion protein Ag3733 |
| Vollständiger Name | interleukin 17A |
| Berechnetes Molekulargewicht | 155 aa, 18 kDa |
| Beobachtetes Molekulargewicht | 18 kDa |
| GenBank-Zugangsnummer | BC067505 |
| Gene symbol | IL-17A |
| Gene ID (NCBI) | 3605 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IL-17A antibody 66148-1-Ig | Protokoll herunterladen |
| IF protocol for IL-17A antibody 66148-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Clin Immunol Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia | ||
Int J Pharm Microneedle system carrying Momordin Ic-loaded ROS-responsive hydrogel ameliorates psoriasis via targeted anti-inflammatory and reactive oxygen species (ROS)-scavenging mechanisms | ||
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
J Integr Med Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9 | ||
Int Immunopharmacol (RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis. | ||
Int Immunopharmacol IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rosa (Verified Customer) (07-08-2022) |
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