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IL-18 Polyklonaler Antikörper

IL-18 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(377)

Kat-Nr. : 10663-1-AP

Synonyme

IL18, Pre-IL18, Iboctadekin, IFN-gamma-inducing factor, IL 1 gamma



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, DU 145-Zellen, HaCaT-Zellen, HeLa-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10663-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA IL-18 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IL-18 fusion protein Ag1063
Vollständiger Name IL-18
Berechnetes Molekulargewicht 193 aa, 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC007461
Gene symbol IL-18
Gene ID (NCBI) 3606
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-18 antibody 10663-1-APProtokoll herunterladen
IF protocol for IL-18 antibody 10663-1-APProtokoll herunterladen
IP protocol for IL-18 antibody 10663-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Sci Adv

FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity.

Authors - Jun Ren
WB

Nat Commun

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Authors - Hung-Yu Chiang
WB

Nat Commun

Telomere dysfunction activates YAP1 to drive tissue inflammation.

Authors - Deepavali Chakravarti
humanWB

J Pineal Res

Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation

Authors - Zitong Wang
WB,IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li
humanWB

Proc Natl Acad Sci U S A

Telomere dysfunction instigates inflammation in inflammatory bowel disease.

Authors - Deepavali Chakravarti

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jillian (Verified Customer) (05-27-2025)

Great antibody, labels very well. We had to titrate multiple times to get to the working value because it had a high background until we reached 1:5000 dilution

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Frontal, ICJ