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IL-18 Polyklonaler Antikörper
IL-18 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10663-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, DU 145-Zellen, HaCaT-Zellen, HeLa-Zellen | 
| Erfolgreiche IP | HeLa-Zellen | 
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below | 
| WB | See 342 publications below | 
| IHC | See 53 publications below | 
| IF | See 35 publications below | 
| IP | See 1 publications below | 
Produktinformation
10663-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA IL-18 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | IL-18 fusion protein Ag1063 | 
| Vollständiger Name | IL-18 | 
| Berechnetes Molekulargewicht | 193 aa, 22 kDa | 
| Beobachtetes Molekulargewicht | 22 kDa | 
| GenBank-Zugangsnummer | BC007461 | 
| Gene symbol | IL-18 | 
| Gene ID (NCBI) | 3606 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IL-18 antibody 10663-1-AP | Protokoll herunterladen | 
| IF protocol for IL-18 antibody 10663-1-AP | Protokoll herunterladen | 
| IP protocol for IL-18 antibody 10663-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Sci Adv FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity. | ||
| Nat Commun IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. | ||
| J Pineal Res Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation | ||
| Aging Dis Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice. | ||
| Proc Natl Acad Sci U S A Telomere dysfunction instigates inflammation in inflammatory bowel disease. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Jillian (Verified Customer) (05-27-2025) | Great antibody, labels very well. We had to titrate multiple times to get to the working value because it had a high background until we reached 1:5000 dilution 
 | 







