IL-18R1/CD218a Rekombinanter Antikörper

IL-18R1/CD218a Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241673G6

Kat-Nr. : 84390-5-RR

Synonyme

IL18R1, IL 18R1, IL 18R 1, EC:3.2.2.6, CD218a

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  WB analysis of NK-92 using 84390-5-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84390-5-RR (IL18R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NK-92 using 84390-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using IL-18R1/CD218a antibody (84390-5-RR, Clone: 241673G6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84390-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84390-5-RR against Human IL-18R1/CD218a were performed. The affinity constant is 0.948 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells
• Erfolgreiche Detektion in IF/ICC: NK-92 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84390-5-RR bindet in WB, IF/ICC, ELISA IL-18R1/CD218a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 18 receptor 1
• Berechnetes Molekulargewicht: 541 aa, 62 kDa
• Beobachtetes Molekulargewicht: 60-75 kDa
• GenBank-Zugangsnummer: BC093975
• Gene symbol: IL18R1
• Gene ID (NCBI): 8809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-18R1 (CD218a) is a component of the interleukin-18 receptor (IL-18R) complex, which is critical for the signaling of IL-18, a pro-inflammatory cytokine. This receptor complex, consisting of IL-18R1 and the IL-18 receptor accessory protein (IL-18RAP), belongs to the immunoglobulin-like family of interleukin-1 receptors (PMID: 35461924). IL-18R1 plays a significant role in inducing the production of interferon-gamma (IFN-γ) and other inflammatory cytokines, contributing to innate and adaptive immune responses (PMID: 2550053). It is also suggested to play a role in hypertension and chronic kidney disease, where the IL-18/IL-18R1 signaling axis may have diagnostic and therapeutic potential (PMID: 35461924).