IL-1 beta Rekombinanter Antikörper

IL-1 beta Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G9

Kat-Nr. : 82696-8-PBS

Synonyme

2G9, Catabolin, IL 1, IL 1 beta, IL1 beta

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  Sandwich Sample test of MP00331-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA or 10 μg/mL PHA and 10 ng/mL IL-2 for 1 day. The mean IL-1 beta concentration was not determined in unstimulated PBMC supernatant, 5,709.3 pg/mL in PHA stimulated PBMC supernatant and 1,110.8 pg/mL in PHA and IL-2 stimulated PBMC supernatant.

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  Sandwich Sample test of MP00331-2

  Serum of four individual healthy human donors was measured. The IL-1 beta concentration of detected samples was determined to be 93.4 pg/mL with a range of 20.3 - 131.0 pg/mL.

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  Sandwich ELISA standard curve of MP80001-6

  Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-PBS. Detection antibody: 66737-1-PBS. Standard:HZ-1164. Range: 15.6-1000 pg/mL.

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  Sandwich ELISA standard curve of MP00331-2

  Sandwich ELISA standard curve of MP00331-2, Human IL-1 beta Recombinant Matched Antibody Pair - PBS only. 82696-15-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0286. 82696-8-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

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  Cytometric bead array standard curve of MP00331-2

  Cytometric bead array standard curve of MP00331-2, IL-1 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82696-15-PBS. Detection antibody: 82696-8-PBS. Standard: Eg0286. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 82696-8-PBS

  Biolayer interferometry (BLl) kinetic assay of 82696-8-PBS against Human IL-1 beta was performed. The affinity constant is 3.63 nM.

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Geprüfte Anwendungen

Produktinformation

82696-8-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Eg0286
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.