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IL-1R1 Rekombinanter Antikörper

IL-1R1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240130A3

Kat-Nr. : 83420-1-PBS

Synonyme

IL1R1, IL 1R 1, EC:3.2.2.6, D2S1473, CD121A

Filter:
  • Western Blot (WB) analysis of various lysates using IL-1R1 Recombinant antibody (83420-1-RR)

    WB analysis using 83420-1-RR (same clone as 83420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83420-1-RR (IL1R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83420-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83420-1-RR

    Affinity and Kinetic Characterization of 83420-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83420-1-RR against Human IL1R1 were performed. The affinity constant is 8.43 nM.

Geprüfte Anwendungen

Produktinformation

83420-1-PBS bindet in WB, ELISA IL-1R1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IL-1R1 fusion protein Ag12602
Vollständiger Name interleukin 1 receptor, type I
Berechnetes Molekulargewicht 569 aa, 65 kDa
Beobachtetes Molekulargewicht55-60 kDa
GenBank-ZugangsnummerBC075063
Gene symbol IL1R1
Gene ID (NCBI) 3554
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).