IL-2 Monoklonaler Antikörper

IL-2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

2F11C5

Rezensionen (1)

Kat-Nr. : 60306-1-Ig

Synonyme

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 60306-1-Ig

  Jurkat cells and human white blood cells (WBC) were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60306-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human tonsillitis using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human breast cancer using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human breast cancer using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of human tonsillitis using 60306-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60306-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

60306-1-Ig bindet in WB, IHC, IF, ELISA IL-2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-2 fusion protein Ag10199
• Vollständiger Name: interleukin 2
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC070338
• Gene symbol: IL2
• Gene ID (NCBI): 3558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Publikationen

Species	Application	Title
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	PLoS One; N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway.; Authors - Chengfen Wang
mouse	WB	Inflammation; Links Between Gut Dysbiosis and Neurotransmitter Disturbance in Chronic Restraint Stress-Induced Depressive Behaviours: the Role of Inflammation; Authors - Hai‑long Yang
pig	WB,IHC	Food Addit Contam Part A Chem Anal; FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.; Authors - Quancheng Liu
human	IHC	J Clin Exp Hematop; Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms; Authors - Haruko Takizawa
human	WB	Front Immunol; PTPRC promoted CD8+ T cell mediated tumor immunity and drug sensitivity in breast cancer: based on pan-cancer analysis and artificial intelligence modeling of immunogenic cell death-based drug sensitivity stratification; Authors - Pengping Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Alex (Verified Customer) (10-11-2019)

IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Colon

IL-2 Antibody Immunohistochemistry, validation (1:2000 dilution) in Mouse Colon (Cat no:60306-1-Ig)