IL-2 Rekombinanter Antikörper

IL-2 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240264B2

Kat-Nr. : 82626-2-PBS

Synonyme

Il2, interleukin 2

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  Sandwich ELISA standard curve of MP00535-3

  Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00535-3

  Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

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  Sample test of MP00535-3

  Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

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  Affinity and Kinetic Characterization of 82626-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82626-2-PBS against Rat IL-2 was performed. The affinity constant is 1.72 nM.

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Geprüfte Anwendungen

Produktinformation

82626-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test IL-2 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-2 fusion protein Eg0260
• Vollständiger Name: interleukin 2
• Berechnetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_053836
• Gene symbol: Il2
• Gene ID (NCBI): 116562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.