IL-20 Polyklonaler Antikörper

IL-20 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18104-1-AP

Synonyme

Cytokine Zcyto10, IL 20, IL20, interleukin 20, ZCYTO10

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  WB analysis of HEK-293 using 18104-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18104-1-AP (IL20 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 18104-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18104-1-AP (IL20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18104-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18104-1-AP (IL20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

18104-1-AP bindet in WB, IF, IHC, ELISA IL-20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-20 fusion protein Ag12447
• Vollständiger Name: interleukin 20
• Berechnetes Molekulargewicht: 406 aa, 44 kDa
• GenBank-Zugangsnummer: BC074949
• Gene symbol: IL-20
• Gene ID (NCBI): 50604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 receptor (IL-20R) and IL-20 are expressed in several normal tissue types, including the lung, skin, and kidney. IL-20 is a cytokine structurally related to interleukin 10. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 can stimulate STAT3 activation in keratinocytes. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells. IL-20 is highly associated with potent inflammatory diseases such as psoriasis, contact hypersensitivity, rheumatoid arthritis, and atherosclerosis.

Publikationen

Species	Application	Title
mouse	IF	Immunology; IL-20 is involved in obesity by modulation of adipogenesis and macrophage dysregulation.; Authors - Yu-Hsiang Hsu