IL-23R Polyklonaler Antikörper

IL-23R Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 27163-1-AP

Synonyme

IL23R, Interleukin-23 receptor, interleukin 23 receptor, IL-23 receptor, IL 23R

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  WB analysis using 27163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27163-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27163-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, Raji-Zellen, RAW 264.7-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

27163-1-AP bindet in WB, IHC, ELISA IL-23R und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-23R fusion protein Ag25939
• Vollständiger Name: interleukin 23 receptor
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: NM_144701
• Gene symbol: IL-23R
• Gene ID (NCBI): 149233
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-23 receptor (IL-23R) is a type I cytokine receptor that plays a critical role in immune responses and inflammation. It forms a receptor complex with IL-12Rβ1 and is activated by the cytokine IL-23, which is primarily produced by dendritic cells and activated macrophages (PMID: 39796684). The genetic and epigenetic interactions in the IL-23R/IL-17 axis are associated with elevated expression of IL-17 and inflammatory bowel disease (IBD) pathogenesis (PMID: 21672939).

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.; Authors - Fengming Ding
rat	WB	Naunyn Schmiedebergs Arch Pharmacol; Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury.; Authors - Wenting Liu
rat	IHC	Int J Biol Macromol; The effects of Cordyceps polysaccharides on ischemic brain injury in rats via intervening with IL-23/IL-17 axis and the intestinal barrier; Authors - Yang Jiang
mouse	WB	Biomaterials; An approach for psoriasis of microneedle patch simultaneously targeting multiple inflammatory cytokines and relapse related T cells; Authors - Li Huang