IL-24 Polyklonaler Antikörper

IL-24 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12064-1-AP

Synonyme

C49A, FISP, IL 24, IL10B, IL24, IL-24, interleukin 24, mda 7, MDA7, Mob 5, ST16

---

Galerie der Validierungsdaten

• 

  WB analysis of Recombinant protein using 12064-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12064-1-AP (IL-24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens)..

• 

  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12064-1-AP bindet in WB, IHC, ELISA IL-24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-24 fusion protein Ag2701
• Vollständiger Name: interleukin 24
• Berechnetes Molekulargewicht: 207 aa, 24 kDa
• GenBank-Zugangsnummer: BC009681
• Gene symbol: IL24
• Gene ID (NCBI): 11009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publikationen

Species	Application	Title
		Nat Med; Glycemic control in diabetes is restored by therapeutic manipulation of cytokines that regulate beta cell stress.; Authors - Sumaira Z Hasnain
human	WB	Front Oncol; Combination Therapy of Prostate Cancer by Oncolytic Adenovirus Harboring Interleukin 24 and Ionizing Radiation.; Authors - Li-Jun Mao
human	WB	J Exp Clin Cancer Res; Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells.; Authors - Jichen Li