IL-24 Polyklonaler Antikörper

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• KD/KO Validated

IL-24 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 26772-1-AP

Synonyme

IL24, Melanoma differentiation-associated gene 7 protein, Interleukin-24, interleukin 24, IL 24

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  WB analysis using 26772-1-AP

  Mouse spleen tissues were subjected to SDS PAGE followed by western blot with 26772-1-AP (IL-24 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

26772-1-AP bindet in WB, IHC, IF, ELISA IL-24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-24 fusion protein Ag25071
• Vollständiger Name: interleukin 24
• Berechnetes Molekulargewicht: 207 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC009681
• Gene symbol: IL-24
• Gene ID (NCBI): 11009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publikationen

Species	Application	Title
mouse	IHC,IF	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li
human	WB	Neoplasia; ZBTB7A Enhances Osteosarcoma Chemoresistance by Transcriptionally Repressing lncRNALINC00473-IL24 Activity.; Authors - Lu Zhang
human	WB	Acta Pharmacol Sin; Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway; Authors - Ji-Xian Xiong ; KD Validated
human	WB	Cell Biol Int; Serum IL-24 combined with CA125 as screening and prognostic biomarkers for NSCLC; Authors - Kai Zhang
human	IF	Exp Mol Med; Single-cell RNA sequencing and spatial transcriptomics of esophageal squamous cell carcinoma with lymph node metastases; Authors - Wei Guo