IL-27A p28 Monoklonaler Antikörper

IL-27A p28 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1E1H5

Kat-Nr. : 66164-1-Ig

Synonyme

IL27, IL-27, IL 27, IL 27 A, IL 27 subunit alpha

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  WB analysis of Recombinant protein using 66164-1-Ig

  IL27 fusion protein were subjected to SDS PAGE followed by western blot with 66164-1-Ig (IL-27 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein, Ag21436

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66164-1-Ig bindet in WB, IHC, ELISA IL-27A p28 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-27A p28 fusion protein Ag21436
• Vollständiger Name: interleukin 27
• Berechnetes Molekulargewicht: 243 aa, 27 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC062422
• Gene symbol: IL-27
• Gene ID (NCBI): 246778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

Publikationen

Species	Application	Title
human	IHC	Anticancer Res; The Prognostic Significance of Peritumoral Lymphocytes' Band-like Structure in Type II Endometrial Cancer.; Authors - Yusuke Shimizu