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CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240906A12

Kat-Nr. : 83896-1-PBS

Synonyme

CD25, 240906A12, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

Filter:
  • Western Blot (WB) analysis of MJ cells using CD25/IL-2RA Recombinant antibody (83896-1-RR)

    WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83896-1-RR

    Affinity and Kinetic Characterization of 83896-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

Geprüfte Anwendungen

Produktinformation

83896-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA CD25/IL-2RA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD25/IL-2RA fusion protein Eg0408
Vollständiger Name interleukin 2 receptor, alpha
Berechnetes Molekulargewicht 272 aa, 31 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).