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CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240906E5

Kat-Nr. : 83896-5-PBS

Synonyme

CD25, 240906E5, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

Filter:
  • Sandwich ELISA standard curve of MP00837-3

    Sandwich ELISA standard curve of MP00837-3

    Sandwich ELISA standard curve of MP00837-3, Human CD25/IL-2RA Recombinant Matched Antibody Pair - PBS only. 83896-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0408. 83896-6-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Sample test of MP00837-3

    Sample test of MP00837-3

    Serum of eight individual healthy human donors was measured. The human CD25/IL-2RA concentration of detected samples was determined to be 993.9 pg/mL with a range of 567.3 - 1,718.0 pg/mL.

  • Sample test of MP00837-3

    Sample test of MP00837-3

    Human peripheral blood mononuclear cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human CD25/IL-2RA.

Geprüfte Anwendungen

Produktinformation

83896-5-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test CD25/IL-2RA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name interleukin 2 receptor, alpha
Berechnetes Molekulargewicht 272 aa, 31 kDa
GenBank-ZugangsnummerBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.