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CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240906D7

Kat-Nr. : 83896-6-PBS

Synonyme

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  • Sandwich ELISA sample test of MP00837-3

    Sandwich ELISA sample test of MP00837-3

    Serum of eight individual healthy human donors was measured. The human CD25/IL-2RA concentration of detected samples was determined to be 993.9 pg/mL with a range of 567.3 - 1,718.0 pg/mL.

  • Sandwich ELISA sample test of MP00837-3

    Sandwich ELISA sample test of MP00837-3

    Human peripheral blood mononuclear cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human CD25/IL-2RA.

  • Sandwich ELISA standard curve of MP00837-3

    Sandwich ELISA standard curve of MP00837-3

    Sandwich ELISA standard curve of MP00837-3, Human CD25/IL-2RA Recombinant Matched Antibody Pair - PBS only. 83896-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0408. 83896-6-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Affinity and Kinetic Characterization of 83896-6-PBS

    Affinity and Kinetic Characterization of 83896-6-PBS

    Biolayer interferometry (BLl) kinetic assay of 83896-6-PBS against Human CD25/IL-2RA was performed. The affinity constant is 0.853 nM.

Geprüfte Anwendungen

Produktinformation

83896-6-PBS bindet in Sandwich ELISA, Indirect ELISA CD25/IL-2RA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD25/IL-2RA fusion protein Eg0408
Vollständiger Name interleukin 2 receptor, alpha
Berechnetes Molekulargewicht 272 aa, 31 kDa
GenBank-ZugangsnummerBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.