CD122/IL-2RB Polyklonaler Antikörper

CD122/IL-2RB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13602-1-AP

Synonyme

CD122, CD122/IL-2RB, IL 2 receptor subunit beta, IL 2R subunit beta, IL 2RB, IL2RB, IL-2RB, interleukin 2 receptor, beta, P70 75, p75

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  WB analysis using 13602-1-AP

  NK-92 cells were subjected to SDS PAGE followed by western blot with 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 13602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

13602-1-AP bindet in WB, IHC, IF, ELISA CD122/IL-2RB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD122/IL-2RB fusion protein Ag3929
• Vollständiger Name: interleukin 2 receptor, beta
• Berechnetes Molekulargewicht: 551 aa, 61 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC025691
• Gene symbol: IL2RB
• Gene ID (NCBI): 3560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.

Publikationen

Species	Application	Title
mouse	WB	Int J Biol Macromol; Activation of IL-2/IL-2R pathway by Hedyotis diffusa polysaccharide improves immunotherapy in colorectal cancer; Authors - Liangliang Bai
Human	WB	Allergol Immunopathol (Madr); IL2RB affects Th1/Th2 and Th17 responses of peripheral blood mononuclear cells from septic patients; Authors - Jiaqian Zhou
mouse	IF	Ocul Surf; Conditional deletion of CD25 in the corneal epithelium reveals sex differences in barrier disruption; Authors - Anmar Abu-Romman
human	WB	J Dermatol Sci; Exosomes containing miR-1469 regulate natural killer cells by targeting CD122 in non-segmental vitiligo; Authors - Yujia Wei
rat	WB	ACS Omega; Vanillic Acid Protects PC12 Cells from Corticosterone-Induced Neurotoxicity via Regulating Immune and Metabolic Dysregulation Based on Computational Metabolomics; Authors - Tianxing Lv