IL2RG Polyklonaler Antikörper

IL2RG Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11409-1-AP

Synonyme

CD132, IL 2 receptor subunit gamma, IL 2R subunit gamma, IL 2RG, IL2RG, IL-2RG, IMD4, p64, SCIDX, SCIDX1

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Galerie der Validierungsdaten

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  WB analysis using 11409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11409-1-AP (IL2RG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 11409-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug IL2RG antibody (11409-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11409-1-AP bindet in WB, FC, ELISA IL2RG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL2RG fusion protein Ag1911
• Vollständiger Name: interleukin 2 receptor, gamma (severe combined immunodeficiency)
• Berechnetes Molekulargewicht: 369 aa, 42 kDa
• Beobachtetes Molekulargewicht: 64-70 kDa
• GenBank-Zugangsnummer: BC014972
• Gene symbol: IL2RG
• Gene ID (NCBI): 3561
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL2RA), beta (IL2RB), and gamma (IL2RG). IL2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.