IL-3 Polyklonaler Antikörper

IL-3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17835-1-AP

Synonyme

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein, Rekombinantes Protein Protein
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17835-1-AP bindet in WB, IHC, ELISA IL-3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-3 fusion protein Ag11839
• Vollständiger Name: interleukin 3 (colony-stimulating factor, multiple)
• Berechnetes Molekulargewicht: 152 aa, 17 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC066273
• Gene symbol: IL3
• Gene ID (NCBI): 3562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Publikationen

Species	Application	Title
pig	WB	JTCVS Open; Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium; Authors - Dwight D Harris