IL-31 Polyklonaler Antikörper

IL-31 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22859-1-AP

Synonyme

IL 31, IL31, IL-31, interleukin 31

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  IHC staining of human tonsillitis using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 22859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

22859-1-AP bindet in IHC, ELISA IL-31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-31 fusion protein Ag18848
• Vollständiger Name: interleukin 31
• Berechnetes Molekulargewicht: 164 aa, 18 kDa
• GenBank-Zugangsnummer: BC132998
• Gene symbol: IL-31
• Gene ID (NCBI): 386653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.

Publikationen

Species	Application	Title
human	IHC	Sci Rep; Interleukin-31 expression and relation to disease severity in human asthma.; Authors - Tianwen Lai
human	IHC,ELISA	World J Gastrointest Oncol; Clinical implications of interleukins-31, 32, and 33 in gastric cancer; Authors - Qing-Hua Liu