IL3RA/CD123 Rekombinanter Antikörper

IL3RA/CD123 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240042E5

Kat-Nr. : 83243-3-PBS

Synonyme

IL3RA, 240042E5, CD123, hIL 3Ra, IL 3 receptor subunit alpha

---

• 

  WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00176-4

  Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

• 

  Sample test of MP00176-4

  THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

• 

  Affinity and Kinetic Characterization of 83243-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83243-3-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

---

Geprüfte Anwendungen

Produktinformation

83243-3-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test IL3RA/CD123 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL3RA/CD123 fusion protein Ag4452
• Vollständiger Name: interleukin 3 receptor, alpha (low affinity)
• Berechnetes Molekulargewicht: 378 aa, 43 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC035407
• Gene symbol: CD123
• Gene ID (NCBI): 3563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.