IL3RA/CD123 Rekombinanter Antikörper

IL3RA/CD123 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

241268E3

Kat-Nr. : Biotin-FcA98107

Synonyme

IL3RA, CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha

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  FC experiment of human PBMCs using Biotin-FcA98107

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1), and 0.25 ug Biotin Anti-Human IL3RA/CD123 Rabbit RecAb (Biotin-FcA98107, Clone: 241268E3) or Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-FcA98107

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human IL3RA/CD123 Rabbit RecAb (Biotin-FcA98107, Clone: 241268E3) (red) or Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-FcA98107 bindet in FC IL3RA/CD123 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 3 receptor, alpha (low affinity)
• Berechnetes Molekulargewicht: 378 aa, 43 kDa
• GenBank-Zugangsnummer: BC035407
• Gene symbol: CD123
• Gene ID (NCBI): 3563
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.