IL-4 Rekombinanter Antikörper

IL-4 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240794D7

Kat-Nr. : 83894-2-PBS

Synonyme

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

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  Sandwich ELISA standard curve of MP00829-3

  Sandwich ELISA standard curve of MP00829-3, Human IL-4 Recombinant Matched Antibody Pair - PBS only. 83894-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0104. 83894-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

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  Sample test of MP00829-3

  Serum of sixteen individual healthy human donors was measured. The human IL-4 concentration of detected samples was determined to be 7.97 pg/mL with a range of 2.25 - 19.49 pg/mL.

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  Sample test of MP00829-3

  Human peripheral blood mononuclear cells (0.5 x 10^6 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 5 μg/mL PHA, and 10 ng/mL recombinant human IL-2. After 7 days of culture, cells were changed into DMEM with 10% fetal bovine serum, 10 ng/mL PMA, 1 μg/mL Ca2+ ionophore, and 5 μg/mL PHA. Cell culture supernate was collected after 1d of stimulation and measured 292.5 pg/mL in stimulated PBMC supernatant, 11.2 pg/mL in unstimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP00829-3

  Cytometric bead array standard curve of MP00829-3, IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83894-2-PBS. Detection antibody: 83894-5-PBS. Standard: Eg0104. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83894-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 83894-2-PBS against Human IL-4 were performed. The affinity constant is 0.434 nM.

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Geprüfte Anwendungen

Produktinformation

83894-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test IL-4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-4 fusion protein Eg0104
• Vollständiger Name: interleukin 4
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• GenBank-Zugangsnummer: BC070123
• Gene symbol: IL-4
• Gene ID (NCBI): 3565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.