IL-4RA/CD124 Monoklonaler Antikörper

IL-4RA/CD124 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E4C11

Kat-Nr. : 67051-1-Ig

Synonyme

IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha

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  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MG-63 using 67051-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Daudi-Zellen, HUVEC-Zellen, Jurkat-Zellen, Raji-Zellen, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67051-1-Ig bindet in WB, ELISA IL-4RA/CD124 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-4RA/CD124 fusion protein Ag28411
• Vollständiger Name: interleukin 4 receptor
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_000418
• Gene symbol: IL-4R
• Gene ID (NCBI): 3566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.