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IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240670B3

Kat-Nr. : 83747-7-PBS

Synonyme

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Filter:
  • Western Blot (WB) analysis of HUVEC cells using IL-6 Recombinant antibody (83747-7-RR)

    WB analysis of HUVEC using 83747-7-RR (same clone as 83747-7-PBS)

    LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83747-7-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83747-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83747-7-RR

    Affinity and Kinetic Characterization of 83747-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83747-7-RR against Human IL-6 were performed. The affinity constant is 1.72 nM.

Geprüfte Anwendungen

Produktinformation

83747-7-PBS bindet in WB, ELISA IL-6 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name interleukin 6 (interferon, beta 2)
Berechnetes Molekulargewicht 212 aa, 24 kDa
Beobachtetes Molekulargewicht20 kDa
GenBank-ZugangsnummerBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.