CD126/IL-6R alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD126/IL-6R alpha Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(47)

Rezensionen (1)

Kat-Nr. : 23457-1-AP

Synonyme

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

---

• 

  WB analysis using 23457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23457-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 23457-1-AP

  IP result of anti-CD126/IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug.

• 

  IHC staining of mouse spleen using 23457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 23457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Jurkat-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below

Produktinformation

23457-1-AP bindet in WB, IHC, IF, IP, ELISA CD126/IL-6R alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD126/IL-6R alpha fusion protein Ag18263
• Vollständiger Name: interleukin 6 receptor
• Berechnetes Molekulargewicht: 468 aa, 52 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC132684
• Gene symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Publikationen

Species	Application	Title
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
mouse	WB	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
human	IF	J Med Virol; Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis; Authors - Arun Das
mouse,human	IF	Acta Pharmacol Sin; Exosomes derived from induced cardiopulmonary progenitor cells alleviate acute lung injury in mice; Authors - Luo-Xing Xia
rat	WB	Int J Biol Macromol; A dual gene-activated dermal scaffolds loaded with nanocomposite particles expressing of VEGF and aFGF: Promoting wound healing by early vascularization; Authors - Jialiang Wang
mouse	WB	Allergol Int; Prevention of IL-6 signaling ameliorates toluene diisocyanate-induced steroid-resistant asthma.; Authors - Shuyu Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Jianhua (Verified Customer) (04-02-2025)

works very well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human T-cells