CD126/IL-6R alpha Rekombinanter Antikörper

CD126/IL-6R alpha Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240734B6

Kat-Nr. : 83688-2-PBS

Synonyme

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

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  Sandwich ELISA standard curve of MP00676-1

  Sandwich ELISA standard curve of MP00676-1, Human IL6R/IL-6R alpha Recombinant Matched Antibody Pair - PBS only. 83688-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0490. 83688-2-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Sample test of MP00676-1

  Serum of sixteen humans was measured. The human IL6R/IL-6R alpha concentration of these samples was determined to be 60,089 pg/mL, with a range of 37,850.6 - 124,335.2 pg/mL.

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  Sample test of MP00676-1

  Urine of eight humans was measured. The human IL6R/IL-6R alpha concentration of these samples was determined to be 1,291.5 pg/mL, with a range of 119.4 - 5,099.9 pg/mL.

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  Sample test of MP00676-1

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1day, 4 days and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL6.

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  Cytometric bead array standard curve of MP00676-1

  Cytometric bead array standard curve of MP00676-1, CD126/IL-6R alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83688-3-PBS. Detection antibody: 83688-2-PBS. Standard: Eg0490. Range: 0.625-80 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83688-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD126/IL-6R alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD126/IL-6R alpha fusion protein Eg0490
• Vollständiger Name: interleukin 6 receptor
• Berechnetes Molekulargewicht: 468 aa, 52 kDa
• GenBank-Zugangsnummer: BC132684
• Gene symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.