CD127/IL-7R Polyklonaler Antikörper

CD127/IL-7R Polyklonal Antikörper für FC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 17626-1-AP

Synonyme

CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor

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Galerie der Validierungsdaten

• 

  WB analysis of mouse thymus using 17626-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17626-1-AP (CD127/IL7R antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17626-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17626-1-AP (CD127/IL7R antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17626-1-AP

  IP Result of anti-CD127/IL7R (IP:17626-1-AP, 4ug; Detection:17626-1-AP 1:1000) with K-562 cells lysate 1200ug.

• 

  FC experiment of K-562 using 17626-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CD127/IL7R antibody (17626-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17626-1-AP bindet in WB, IP, FC, ELISA CD127/IL-7R und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD127/IL-7R fusion protein Ag11844
• Vollständiger Name: interleukin 7 receptor
• Berechnetes Molekulargewicht: 459 aa, 52 kDa
• Beobachtetes Molekulargewicht: 56-58 kDa
• GenBank-Zugangsnummer: BC067537
• Gene symbol: IL7R
• Gene ID (NCBI): 3575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Publikationen

Species	Application	Title
human,mouse	WB	J Mammary Gland Biol Neoplasia; TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties; Authors - Nikolina Giotopoulou
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bastien (Verified Customer) (08-19-2020)

Western blotting of protein from B cells of mice spleen. Protein extract was loaded on 8 % polyacrylamide gel and then transfert on nitrocellulose membrane. Nitrocellulose membrane was blocked with 5% BSA during 1h. Membrane was then incubated overnight at 4°C with 1/500 IL7Ra antibody (17626-1-AP). Finally, the membrane was incubated with anti-rabbit-HRP (clean blot IPdetection reagent from thermofisher)

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: B cells

CD127/IL-7R Antibody Western Blot validation (1/500 dilution) in B cells (Cat no:17626-1-AP)

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