CXCR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCR2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 20634-1-AP

Synonyme

CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, IL-8RB, interleukin 8 receptor, beta

---

Galerie der Validierungsdaten

• 

  WB analysis of L02 using 20634-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20634-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20634-1-AP

  IP Result of anti-CXCR2 (IP:20634-1-AP, 4ug; Detection:20634-1-AP 1:1000) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20634-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL8RB antibody 20634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, COLO 320-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below

Produktinformation

20634-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CXCR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCR2 fusion protein Ag14670
• Vollständiger Name: interleukin 8 receptor, beta
• Berechnetes Molekulargewicht: 360 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC037961
• Gene symbol: IL8RB
• Gene ID (NCBI): 3579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA, and NAP-2.

Publikationen

Species	Application	Title
human	IF	Cell Rep; Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8+ T cell; Authors - Fan Xu
human	WB	Sci Signal; Skewing cPLA2α activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis; Authors - Kenneth D Maus
human	WB	Int J Mol Sci; MyD88 in Macrophages Enhances Liver Fibrosis by Activation of NLRP3 Inflammasome in HSCs.; Authors - Shuang Ge
human	IF	Front Immunol; CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion; Authors - Sainan Zhang
human	WB,IHC,IF	Pharmaceuticals (Basel); CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway; Authors - Hai-Zhou Ji ; KD Validated
human	IF	JCI Insight; Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.; Authors - Charli Dominguez

Verwandte Produkte

CXCR1 Polyclonal antibody

55450-1-AP

CXCL8/IL-8 Monoclonal antibody

60141-1-Ig

CXCL1 Polyclonal antibody

12335-1-AP

CXCR3B-specific Monoclonal antibody

60065-1-Ig