ILK Rekombinanter Antikörper

ILK Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242982A5

Kat-Nr. : 85428-5-PBS

Synonyme

59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, EC:2.7.11.1, ILK 1, ILK 2

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  WB analysis using 85428-5-RR (same clone as 85428-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85428-5-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 85428-5-RR (same clone as 85428-5-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85428-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85428-5-RR (same clone as 85428-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ILK antibody (85428-5-RR, Clone: 242982A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85428-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85428-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85428-5-RR against Human ILK were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85428-5-PBS bindet in WB, IF/ICC, Indirect ELISA ILK und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ILK fusion protein Ag3570
• Vollständiger Name: integrin-linked kinase
• Berechnetes Molekulargewicht: 451 aa, 59 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC001554
• Gene symbol: ILK
• Gene ID (NCBI): 3611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).