IMMT Monoklonaler Antikörper

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IMMT Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D2E8

Kat-Nr. : 68226-1-PBS

Synonyme

MINOS2, MICOS complex subunit MIC60, MIC60, HMP, Cell proliferation-inducing gene 4/52 protein

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  WB analysis using 68226-1-Ig (same clone as 68226-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68226-1-Ig (IMMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

  WB result of IMMT antibody (68226-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMMT transfected HeLa cells. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 68226-1-Ig (same clone as 68226-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IMMT (68226-1-Ig, Clone:2D2E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50515-1

  Cytometric bead array standard curve of MP50515-1, IMMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68226-1-PBS. Detection antibody: 68226-2-PBS. Standard:Ag0102. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50515-2

  Cytometric bead array standard curve of MP50515-2, IMMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68226-3-PBS. Detection antibody: 68226-1-PBS. Standard:Ag0102. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68226-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA IMMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IMMT fusion protein Ag0102
• Vollständiger Name: inner membrane protein, mitochondrial (mitofilin)
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC002412
• Gene symbol: IMMT
• Gene ID (NCBI): 10989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.