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IMP3 Polyklonaler Antikörper

IMP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12750-1-PBS

Synonyme

MRPS4, IMP 3, C15orf12, BRMS2

Filter:
  • Western Blot (WB) analysis of HeLa cells using IMP3 Polyclonal antibody (12750-1-AP)

    WB analysis of HeLa using 12750-1-AP (same clone as 12750-1-PBS)

    WB result of IMP3 antibody (12750-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMP3 transfected HeLa cells. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IMP3 Polyclonal antibody (12750-1-AP)

    WB analysis using 12750-1-AP (same clone as 12750-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using IMP3 Polyclonal antibody (12750-1-AP)

    IP experiment of HeLa using 12750-1-AP (same clone as 12750-1-PBS)

    IP result of anti-IMP3 (IP:12750-1-AP, 3ug; Detection:12750-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IMP3 Polyclonal antibody (12750-1-AP)

    IHC staining of human ovary cancer using 12750-1-AP (same clone as 12750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12750-1-AP (IMP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMP3 Polyclonal antibody (12750-1-AP)

    IF Staining of HepG2 using 12750-1-AP (same clone as 12750-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (12750-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12750-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA IMP3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IMP3 fusion protein Ag3477
Vollständiger Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Berechnetes Molekulargewicht 184 aa, 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC020557
Gene symbol IMP3
Gene ID (NCBI) 55272
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMP U3 small nucleolar ribonucleoprotein 3 (IMP3, BRMS2), is a component of U3 small nucleolar ribonucleoprotein complex (U3 snoRNP), has been identified as a participant in pre-rRNA processing for nearly twenty years.