IMPDH1 Rekombinanter Antikörper

IMPDH1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242329D8

Kat-Nr. : 84913-1-PBS

Synonyme

242329D8, EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1

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  WB analysis using 84913-1-RR (same clone as 84913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 84913-1-RR (same clone as 84913-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP01726-1

  The mean IMPDH1 concentration was determined to be 27.79 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 45.02 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01726-1

  Sandwich ELISA standard curve of MP01726-1, Human IMPDH1 Recombinant Matched Antibody Pair - PBS only. 84913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17473. 84913-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Affinity and Kinetic Characterization of 84913-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.

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Geprüfte Anwendungen

Produktinformation

84913-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA IMPDH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IMPDH1 fusion protein Ag17473
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 1
• Berechnetes Molekulargewicht: 563 aa, 60 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: BC033622
• Gene symbol: IMPDH1
• Gene ID (NCBI): 3614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa.