IMPDH1 Rekombinanter Antikörper

IMPDH1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242329D8

Kat-Nr. : 84913-1-RR

Synonyme

IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1, EC:1.1.1.205

---

• 

  WB analysis using 84913-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 84913-1-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84913-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84913-1-RR bindet in WB, ELISA IMPDH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IMPDH1 fusion protein Ag17473
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 1
• Berechnetes Molekulargewicht: 563 aa, 60 kDa
• Beobachtetes Molekulargewicht: 53-60 kDa
• GenBank-Zugangsnummer: BC033622
• Gene symbol: IMPDH1
• Gene ID (NCBI): 3614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa.