IMPDH2 Polyklonaler Antikörper

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• KD/KO Validated

IMPDH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12948-1-PBS

Synonyme

EC:1.1.1.205, IMP dehydrogenase II, IMPD 2, IMPD2, IMPDH 2

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  WB analysis of A549 using 12948-1-AP (same clone as 12948-1-PBS)

  WB result of IMPDH2 antibody (12948-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells. This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  WB analysis using 12948-1-AP (same clone as 12948-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 12948-1-AP (same clone as 12948-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12948-1-AP (same clone as 12948-1-PBS)

  IP result of anti-IMPDH2 (IP:12948-1-AP, 4ug; Detection:12948-1-AP 1:20000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12948-1-AP (same clone as 12948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12948-1-AP (same clone as 12948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12948-1-AP (same clone as 12948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12948-1-AP (same clone as 12948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12948-1-AP (same clone as 12948-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMPDH2 antibody (12948-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12948-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12948-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA IMPDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IMPDH2 fusion protein Ag3632
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 2
• Berechnetes Molekulargewicht: 514 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC012840
• Gene symbol: IMPDH2
• Gene ID (NCBI): 3615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may have cross-reaction with IMPDH1 due to the high homology.