IMPDH2 Monoklonaler Antikörper

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• KD/KO Validated

IMPDH2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1B1G8

Kat-Nr. : 67663-1-Ig

Synonyme

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

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  WB analysis using 67663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 67663-1-Ig

  WB result of IMPDH2 antibody (67663-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells.

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  WB analysis using 67663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67663-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67663-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using IMPDH2 antibody (67663-1-Ig, Clone: 1B1G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67663-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA IMPDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IMPDH2 fusion protein Ag17170
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 2
• Berechnetes Molekulargewicht: 514 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC012840
• Gene symbol: IMPDH2
• Gene ID (NCBI): 3615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB	Epigenomics; Epigenetic silencing of KCTD8 promotes hepatocellular carcinoma growth by activating PI3K/AKT signaling; Authors - Jing Zhou ; KD Validated
human	WB,IHC,IF,CoIP	J Exp Clin Cancer Res; Hypoxia-induced TMTC3 expression in esophageal squamous cell carcinoma potentiates tumor angiogenesis through Rho GTPase/STAT3/VEGFA pathway; Authors - Hongyu Yuan ; KD Validated