IMPDH2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

IMPDH2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B1G8

Kat-Nr. : 67663-1-PBS

Synonyme

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

---

• 

  WB analysis using 67663-1-Ig (same clone as 67663-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67663-1-Ig (same clone as 67663-1-PBS)

  WB result of IMPDH2 antibody (67663-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67663-1-Ig (same clone as 67663-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67663-1-Ig (same clone as 67663-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67663-1-Ig (same clone as 67663-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using IMPDH2 antibody (67663-1-Ig, Clone: 1B1G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67663-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67663-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA IMPDH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IMPDH2 fusion protein Ag17170
• Vollständiger Name: IMP (inosine monophosphate) dehydrogenase 2
• Berechnetes Molekulargewicht: 514 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC012840
• Gene symbol: IMPDH2
• Gene ID (NCBI): 3615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270)