IMUP Polyklonaler Antikörper

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• KD/KO Validated

IMUP Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25561-1-AP

Synonyme

C19orf33, H2RSP, HAI 2 related small protein, IMUP, IMUP 1, IMUP 2

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  WB analysis of A549 using 25561-1-AP

  WB result of IMUP antibody (25561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMUP transfected A549 cells.

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  WB analysis using 25561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25561-1-AP (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 25561-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25561-1-AP (C19orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EC109-Zellen, A549-Zellen, HeLa-Zellen, L02-Zellen, SGC-7901-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25561-1-AP bindet in WB, ELISA IMUP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IMUP fusion protein Ag22218
• Vollständiger Name: chromosome 19 open reading frame 33
• Berechnetes Molekulargewicht: 106 aa, 11 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 22 kDa
• GenBank-Zugangsnummer: BC060319
• Gene symbol: IMUP
• Gene ID (NCBI): 64073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). This polyclonal antibody raised against 1-106 aa of human IMUP can recognize both IMUP-1 and IMUP-2. The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).