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IMUP Polyklonaler Antikörper

IMUP Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25561-1-PBS

Synonyme

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  • Western Blot (WB) analysis of A549 cells using IMUP Polyclonal antibody (25561-1-AP)

    WB analysis of A549 using 25561-1-AP (same clone as 25561-1-PBS)

    WB result of IMUP antibody (25561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMUP transfected A549 cells. This data was developed using the same antibody clone with 25561-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IMUP Polyclonal antibody (25561-1-AP)

    WB analysis using 25561-1-AP (same clone as 25561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25561-1-AP (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25561-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using IMUP Polyclonal antibody (25561-1-AP)

    WB analysis of SGC-7901 using 25561-1-AP (same clone as 25561-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25561-1-AP (C19orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25561-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25561-1-PBS bindet in WB, Indirect ELISA IMUP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IMUP fusion protein Ag22218
Vollständiger Name chromosome 19 open reading frame 33
Berechnetes Molekulargewicht 106 aa, 11 kDa
Beobachtetes Molekulargewicht20 kDa, 22 kDa
GenBank-ZugangsnummerBC060319
Gene symbol IMUP
Gene ID (NCBI) 64073
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). This polyclonal antibody raised against 1-106 aa of human IMUP can recognize both IMUP-1 and IMUP-2. The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).