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IMUP Rekombinanter Antikörper

IMUP Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241220B4

Kat-Nr. : 84148-6-PBS

Synonyme

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IMUP Recombinant antibody (84148-6-RR)

    IF Staining of A431 using 84148-6-RR (same clone as 84148-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf33 antibody (84148-6-RR, Clone: 241220B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84148-6-RR

    Affinity and Kinetic Characterization of 84148-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84148-6-RR against Human IMUP were performed. The affinity constant is 10.3 nM.

Geprüfte Anwendungen

Produktinformation

84148-6-PBS bindet in IHC, IF/ICC, Indirect ELISA IMUP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen IMUP fusion protein Ag22218
Vollständiger Name chromosome 19 open reading frame 33
Berechnetes Molekulargewicht 106 aa, 11 kDa
GenBank-ZugangsnummerBC060319
Gene symbol IMUP
Gene ID (NCBI) 64073
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).