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INF2 Monoklonaler Antikörper

INF2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A11A7

Kat-Nr. : 66910-1-PBS

Synonyme

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

Filter:
  • Western Blot (WB) analysis of various lysates using INF2 Monoclonal antibody (66910-1-Ig)

    WB analysis using 66910-1-Ig (same clone as 66910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66910-1-Ig (INF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INF2 Monoclonal antibody (66910-1-Ig)

    IF Staining of HepG2 using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INF2 antibody (66910-1-Ig, Clone: 4A11A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66910-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA INF2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen INF2 fusion protein Ag17043
Vollständiger Name inverted formin, FH2 and WH2 domain containing
Berechnetes Molekulargewicht 1249 aa, 136 kDa
Beobachtetes Molekulargewicht 150-170 kDa
GenBank-ZugangsnummerBC008756
Gene symbol INF2
Gene ID (NCBI) 64423
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).