ING2 Polyklonaler Antikörper

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• KD/KO Validated

ING2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11560-1-PBS

Synonyme

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  WB analysis of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11560-1-AP (same clone as 11560-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  IP result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11560-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ING2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ING2 fusion protein Ag2121
• Vollständiger Name: inhibitor of growth family, member 2
• Berechnetes Molekulargewicht: 280 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC030128
• Gene symbol: ING2
• Gene ID (NCBI): 3622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.