ING4 Polyklonaler Antikörper

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• KD/KO Validated

ING4 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 10617-1-AP

Synonyme

ING4, Inhibitor of growth protein 4, my036, p29ING4

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Galerie der Validierungsdaten

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  WB analysis using 10617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10617-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10617-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10617-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10617-1-AP (ING4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10617-1-AP

  IP Result of anti-ING4 (IP:10617-1-AP, 3ug; Detection:10617-1-AP 1:200) with HEK-293 cells lysate 2700ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

10617-1-AP bindet in WB, IP, IHC, IF, ELISA ING4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ING4 fusion protein Ag0960
• Vollständiger Name: inhibitor of growth family, member 4
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007781
• Gene symbol: ING4
• Gene ID (NCBI): 51147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion. This antibody is a rabbit polyclonal antibody raised against full length human ING4 antigen.

Publikationen

Species	Application	Title
human	WB,IHC,IF,IP	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Blood; The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis.; Authors - Mishima Yuta Y
mouse	IHC	Cancer Res; Transient Induction of ING4 by MYC Drives Prostate Epithelial Cell Differentiation and its Disruption Drives Prostate Tumorigenesis.; Authors - Penny L Berger
human	WB	Oncogene; Aberrant CREB1 activation in prostate cancer disrupts normal prostate luminal cell differentiation.; Authors - M J Watson
human	WB,IHC	Carcinogenesis; Role of ING4 in human melanoma cell migration, invasion and patient survival.; Authors - Li Jun J
human	WB,IHC,IF,IP	Mol Cell Biol; The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes.; Authors - Nozell Susan S