ING5 Polyklonaler Antikörper

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• KD/KO Validated

ING5 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10665-1-PBS

Synonyme

Inhibitor of growth protein 5, p28ING5

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  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10665-1-AP (same clone as 10665-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

  IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10665-1-PBS bindet in WB, IHC, IP, Indirect ELISA ING5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ING5 fusion protein Ag1059
• Vollständiger Name: inhibitor of growth family, member 5
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC005370
• Gene symbol: ING5
• Gene ID (NCBI): 84289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)