- Featured Product
- KD/KO Validated
INO80 Polyklonaler Antikörper
INO80 Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, IF, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18810-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 8 publications below |
| WB | See 14 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| ChIP | See 4 publications below |
Produktinformation
18810-1-AP bindet in WB, IP, IF, ChIP, ELISA INO80 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | INO80 homolog (S. cerevisiae) |
| Berechnetes Molekulargewicht | 1556 aa, 177 kDa |
| Beobachtetes Molekulargewicht | 178 kDa |
| GenBank-Zugangsnummer | BC146785 |
| Gene symbol | INO80 |
| Gene ID (NCBI) | 54617 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for INO80 antibody 18810-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Genet Mammalian SWI/SNF continuously restores local accessibility to chromatin.
| ||
Cell Stem Cell INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.
| ||
Mol Cell Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin. | ||
Mol Cell Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
| ||
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Nat Commun Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.
|
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH G (Verified Customer) (06-13-2022) | INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.
|
