INPP5A Polyklonaler Antikörper

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• KD/KO Validated

INPP5A Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21723-1-AP

Synonyme

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

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  WB analysis of HeLa using 21723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21723-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21723-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21723-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

21723-1-AP bindet in WB, ELISA INPP5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INPP5A fusion protein Ag16380
• Vollständiger Name: inositol polyphosphate-5-phosphatase, 40kDa
• Berechnetes Molekulargewicht: 412 aa, 48 kDa
• Beobachtetes Molekulargewicht: 43-48 kDa
• GenBank-Zugangsnummer: BC096280
• Gene symbol: INPP5A
• Gene ID (NCBI): 3632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.

Publikationen

Species	Application	Title
human	WB	Dev Cell; Functional genetic screen identifies ITPR3/calcium/RELB axis as a driver of colorectal cancer metastatic liver colonization.; Authors - Ryan H Moy ; KD Validated