INPP5B Polyklonaler Antikörper

INPP5B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15141-1-AP

Synonyme

5PTase, INPP5B, Phosphoinositide 5 phosphatase

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 15141-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15141-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15141-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 15141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15141-1-AP (INPP5B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15141-1-AP (INPP5B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, HeLa-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15141-1-AP bindet in WB, IHC, IF, ELISA INPP5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INPP5B fusion protein Ag7315
• Vollständiger Name: inositol polyphosphate-5-phosphatase, 75kDa
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC058932
• Gene symbol: INPP5B
• Gene ID (NCBI): 3633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INPP5B(Type II inositol 1,4,5-trisphosphate 5-phosphatase) is also named as OCRL2,5PTase, 75 kDa inositol polyphosphate-5-phosphatase and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. This protein, originally described in platelets, is one of at least three known enzymes capable of dephosphorylating inositol-1,4,5-trisphosphate (IP3) to inositol-1,4-bisphosphate (IP2)(PMID:8125013). It has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	J Am Soc Nephrol; Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model.; Authors - Megan L Gliozzi
human	WB	Cancer Cell Int; Decreased INPP5B expression predicts poor prognosis in lung adenocarcinoma.; Authors - Jun Deng
human, mouse	WB,IHC	PLoS One; Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe.; Authors - Luo Na N
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages; Authors - Lin Shen
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss