INPP5D Polyklonaler Antikörper

INPP5D Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19694-1-PBS

Synonyme

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  WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 19694-1-AP (same clone as 19694-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Ramos using 19694-1-AP (same clone as 19694-1-PBS)

  IP result of anti-INPP5D (IP:19694-1-AP, 4ug; Detection:19694-1-AP 1:300) with Ramos cells lysate 3600 ug. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19694-1-PBS bindet in WB, IHC, IP, Indirect ELISA INPP5D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: inositol polyphosphate-5-phosphatase, 145kDa
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: NM_001017915
• Gene symbol: INPP5D
• Gene ID (NCBI): 3635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INPP5D, also named as SHIP, SHIP1, SIP-145 and hp51CN, belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. INPP5D is phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. INPP5D acts as a negative regulator of B-cell antigen receptor signaling. It mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. INPP5D acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. INPP5D regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It is involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. It is a key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. It modulates FCGR3/CD16-mediated cytotoxicity in NK cells. It mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. INPP5D may also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. This antibody is specific to INPP5D.