INPP5K Polyklonaler Antikörper

INPP5K Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 15098-1-AP

Synonyme

Phosphatidylinositol-4,5-bisphosphate 5-phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate 5-phosphatase K, EC:3.1.3.86, EC:3.1.3.56

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  WB analysis of HEK-293 using 15098-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15098-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15098-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15098-1-AP (INPP5K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

15098-1-AP bindet in WB, IHC, IF/ICC, ELISA INPP5K und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INPP5K fusion protein Ag7192
• Vollständiger Name: inositol polyphosphate-5-phosphatase K
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45-51 kDa
• GenBank-Zugangsnummer: BC004362
• Gene symbol: INPP5K
• Gene ID (NCBI): 51763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.; Authors - David Furkert
human,mouse	WB,IF	Front Mol Neurosci; Knockdown of INPP5K compromises the differentiation of N2A cells; Authors - Annamaria Manzolillo
mouse	WB	Exp Neurol; METTL5-mediated 18S rRNA m6A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury; Authors - Zhenpeng Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tim (Verified Customer) (06-28-2021)

Detected transfected GFP-tagged INPP5K in Cos7 cells well. Antibody incubated with membrane overnight in fridge.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Cos7

INPP5K Antibody Western Blot validation (1/1000 dilution) in Cos7 (Cat no:15098-1-AP)